Minggu, 24 September 2017

Ebook Download CRISPR-Cas: A Laboratory ManualFrom Cold Spring Harbor Laboratory Press

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CRISPR-Cas: A Laboratory ManualFrom Cold Spring Harbor Laboratory Press

The development of CRISPR-Cas technology is revolutionizing biology. Based on machinery bacteria use to target foreign nucleic acids, these powerful techniques allow investigators to edit nucleic acids and modulate gene expression more rapidly and accurately than ever before.

Featuring contributions from leading figures in the CRISPR-Cas field, this laboratory manual presents a state-of-the-art guide to the technology. It includes step-by-step protocols for applying CRISPR-Cas-based techniques in various systems, including yeast, zebrafish, Drosophila, mice, and cultured cells (e.g., human pluripotent stem cells). The contributors cover web-based tools and approaches for designing guide RNAs that precisely target genes of interest, methods for preparing and delivering CRISPR-Cas reagents into cells, and ways to screen for cells that harbor the desired genetic changes. Strategies for optimizing CRISPR-Cas in each system--especially for minimizing off-target effects--are also provided.

Authors also describe other applications of the CRISPR-Cas system, including its use for regulating genome activation and repression, and discuss the development of next-generation CRISPR-Cas tools. The book is thus an essential laboratory resource for all cell, molecular, and developmental biologists, as well as biochemists, geneticists, and all who seek to expand their biotechnology toolkits.

  • Sales Rank: #1070552 in Books
  • Published on: 2016-03-23
  • Original language: English
  • Number of items: 1
  • Dimensions: 8.50" h x .50" w x 10.80" l, 2.20 pounds
  • Binding: Paperback
  • 192 pages

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Minggu, 06 Agustus 2017

Free Download The Spirit Catches You and You Fall Down: A Hmong Child, Her American Doctors, and the Collision of Two Cultures

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The Spirit Catches You and You Fall Down: A Hmong Child, Her American Doctors, and the Collision of Two Cultures

Product details

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Audible Audiobook

Listening Length: 13 hours and 37 minutes

Program Type: Audiobook

Version: Unabridged

Publisher: Audible Studios

Audible.com Release Date: March 18, 2015

Whispersync for Voice: Ready

Language: English, English

ASIN: B00UZ9BD8E

Amazon Best Sellers Rank:

This was a required textbook for my Honors English Comp class. I read it in one day. Then, I re-read it more slowly. The culture clash was one most of us have never heard of -- who has really heard of the Hmong? I know I certainly didn't. Although I *was* alive during the Vietnam war and had a father who served three tours (Navy) and I actually lived on Guam, I rarely heard of Laos. My heart broke during this book. More than once!My school had the pleasure of having Anne Fadiman speaking almost immediately after our class finished the book. As only two of us actually read it (why take an honors course if you are going to brush it off?) we were introduced to Ms. Fadiman. I told her my favorite part of the book. She beamed at me -- and in her prepared speech, read that very part! My copy is autographed!If you want a true story, one that teaches you about cultures, one that teaches you about the value of FAMILY -- read this book. I know I look at my family in a very different way after reading this book.Even if you DON'T have to read this book for an English class, read it. It will change you forever!

This book was written in 1997 and I first read it in nursing school. Although it’s been almost twenty years, “The Spirit Catches You and You Fall Down” is just as relevant now as it was when it was written. In fact, I somehow mentioned the book to my mother in law and when I found out she hadn’t read it, I bought her a copy for Christmas. I then felt compelled to re-read the book myself, and discovered that I’ve lent out my copy. It’s been long enough that I’m guessing that I’m not getting my copy back- especially since I don’t recall who has my copy! I came to Amazon today to purchase another copy for myself. The book is just that good.Anne Fadiman’s book is really engaging and just draws the reader in. Although “The Spirit Catches You and You Fall Down” touches on so sensitive subjects, Anne Fadiman manages to illustrate the cultural differences so starkly that I ended up feeling sympathetic to both the western medical community that was trying to treat Lia Lee as well as Lia’s family and her community. It’s written far more like a dialogue between author and reader than a narrative.I work as a nurse I often think of this book when I watch a new mom stuff cotton in her ears, put a towel over her head before going outside, reject the hospital food in favor of broth and chicken brought in by family, or politely remind me over and over “No ice in the water please!”. I honestly think this book should be required reading for doctors, nurses, social workers, foster parents, etc.

A real eyeopener to the Hmong culture, the treatment of epilepsy and the various views of medical staff and caregivers. I was drawn to this book because I have a child who has had seizures. Learning the viewpoint of another culture toward epilepsy gave me greater understanding on facing such a disability. It was unsettling to realize the different approaches to treatment and how cultural differences were/are not always recognized (to the benefit of the patient and their family). This was not an enjoyable book to read, but it was also not easy to put down. I hope that more recently cultural awarness in medical staff and caregivers has been recognized and is being put to use.

This was a required book for a psych nursing class. I quite enjoyed it overall and have read it twice now (once for class and once out of class). The author has a unique way of writing, where a couple of chapters talk about the family and then a couple chapters talk about the history of the Hmong people; I will admit about halfway through the book that started to annoy me as I wanted to know what happened with the family, so I went through and found/read the chapters on them, and then went back and found/read the history chapters. Probably not what the author or my instructor intended, but I still retained all the info and got 100% of the test questions on this book correct.Overall, this is an awesome book for understanding cultural differences in the healthcare setting and how a little bit of effort into cultural understanding and language translating could drastically change the outcome for the patient and the family. Read this please if you're going into any medical field, especially if you're going to be working in an area with diverse ethnic populations.

I'm hesitant to review this book because I know I will not do it justice. It was truly amazing; extremely interesting subject matter written about with such eloquence. I loved it!The main story is about a sick little girl, but there's so much more to it. Hmong tradition & history is expertly woven into the pages. Most stories about tribes & clans are written by ppl who go thousands of miles away & come back to America to tell the story. The Hmong came to America & the story unfolded before the eyes of many Americans, some of whom wished it hadn't. It was interesting to see how the Hmong reacted to our own customs & habits, and even more interesting to read about theirs. The girl's first seizure occurred when her sister slammed a door. According to the Hmong, the loud noise scared her soul away, so she got sick. You can also get sick if you point your finger at the full moon, pee on a rock that looks like a tiger, or wash your clothes in a lake w/ dragons.Though it was emotionally intense, it was not a ball-your-eyes-out kind of book. There were a few sad parts, a few funny parts, and even a little fantasy (their shamans rode winged horses through a staircase to the 'sky-world' to bargain for souls of the sick.) And of course, there was a heavy dose of reality; how they were treated when they came here, the best way to bridge the communication gap, welfare programs & the justice system, the concerns of her doctors & care givers. How do you explain pneumonia to ppl who don't know what lungs are!?Overall, an excellent & fascinating book.

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Rabu, 14 Juni 2017

Free Ebook Osprey Military History Calendar 2016 (General Military)By Osprey Publishing

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  • Sales Rank: #811179 in Books
  • Brand: Osprey Publishing
  • Published on: 2015-07-21
  • Released on: 2015-07-21
  • Original language: English
  • Number of items: 1
  • Dimensions: 11.52" h x .16" w x 11.73" l, .31 pounds
  • Binding: Calendar
  • 24 pages

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Senin, 12 Juni 2017

Get Free Ebook Make: Drones: Teach an Arduino to FlyBy David McGriffy

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Make: Drones will help the widest possible audience understand how drones work by providing several DIY drone projects based on the world's most popular robot controller--the Arduino. The information imparted in this book will show Makers how to build better drones and be better drone pilots, and incidentally it will have applications in almost any robotics project. Why Arduino? Makers know Arduinos and their accessories, they are widely available and inexpensive, and there is strong community support. Open source flight-control code is available for Arduino, and flying is the hook that makes it exciting, even magical, for so many people. Arduino is not only a powerful board in its own right, but it's used as the controller of most inexpensive 3d printers, many desktop CNCs, and the majority of open source drone platforms.

  • Sales Rank: #232128 in eBooks
  • Published on: 2016-10-10
  • Released on: 2016-10-10
  • Format: Kindle eBook

About the Author

David McGriffy has been a programmer since the earliest days of the personal computer. He has piloted both radio-controlled and human-carrying aircraft, including gliders of both sorts. He worked on GPS systems before they had even finished launching all the satellites. He's written embedded systems code for top computer companies and for fun. He has traveled the world to do failure-mode analyses on the control systems of offshore oil rigs and is an inventor on a patent for a vibration analysis system. He is an expert on website performance and has been published by IEEE on the subject. David runs an audio software company, VVAudio, which produces plugins for surround sound and virtual reality. He has a Ph.D. in Physics from the University of Texas at Austin.

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Jumat, 26 Mei 2017

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John Locke's LiberalismBy Ruth W. Grant

In this work, Ruth W. Grant presents a new approach to John Locke's familiar works. Taking the unusual step of relating Locke's Two Treatises to his Essay Concerning Human Understanding, Grant establishes the unity and coherence of Locke's political arguments. She analyzes the Two Treatises as a systematic demonstration of liberal principles of right and power and grounds it in the epistemology set forth in the Essay.

  • Sales Rank: #2250558 in Books
  • Published on: 1991-08-27
  • Original language: English
  • Number of items: 1
  • Dimensions: 9.00" h x .80" w x 6.00" l, .60 pounds
  • Binding: Paperback
  • 230 pages

From the Back Cover
In John Locke's Liberalism, Ruth W. Grant takes issue with prevailing contemporary interpretations of John Locke's political thought. Taking the unusual step of relating Locke's Two Treatises to his Essay Concerning Human Understanding, Grant establishes the unity and coherence of Locke's political arguments.

About the Author
Ruth W. Grant is assistant professor of political science at Duke University.

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Senin, 08 Mei 2017

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Accelerator Physics: Example Problems With SolutionsBy William W Mackay

This manual provides solutions to the problems given in the second edition of the textbook entitled An Introduction to the Physics of Particle Accelerators. Simple-to-solve problems play a useful role as a first check of the student's level of knowledge whereas difficult problems will test the student's capacity of finding the bearing of the problems in an interdisciplinary environment. The solutions to several problems will require strong engagement of the student, not only in accelerator physics but also in more general physical subjects, such as the profound approach to classical mechanics (discussed in Chapter 3) and the subtleties of spin dynamics (Chapter 13).

  • Sales Rank: #2115118 in Books
  • Brand: Brand: World Scientific Publishing Company
  • Published on: 2012-03-23
  • Released on: 2012-03-23
  • Original language: English
  • Number of items: 1
  • Dimensions: 9.00" h x .65" w x 6.00" l, .95 pounds
  • Binding: Paperback
  • 288 pages
Features
  • Used Book in Good Condition

Review
"Overall, this book presents an opportunity to develop more-than-a-basic level of understanding of the physics of particle accelerators, and be exposed to a variety of real-world problems. Therefore, I thoroughly recommend it to any student of accelerator physics, particle physics and any reader who requires some knowledge of how these machines really work." -- Contemporary Physics

From the Inside Flap
This manual provides solutions to the problems given in the second edition of the textbook entitled An Introduction to the Physics of Particle Accelerators. Simple-to-solve problems play a useful role as a first check of the student's level of knowledge whereas difficult problems will test the student's capacity of finding the bearing of the problems in an interdisciplinary environment. The solutions to several problems will require strong engagement of the student, not only in accelerator physics but also in more general physical subjects, such as the profound approach to classical mechanics (discussed in Chapter 3) and the subtleties of spin dynamics (Chapter 13).

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Senin, 01 Mei 2017

Download Ebook Destruction: The Distraction Trilogy #2By A. E. Murphy

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Destruction: The Distraction Trilogy #2By A. E. Murphy

Isaac and Eloise thought that the worst was behind them. At last they can stop sneaking around but at what cost? Isaac expected to lose his job and Eloise was glad to be free of her overbearing and controlling father. What they didn't know was just how much they would lose and to Isaac it feels like everything. Life has to go on and Eloise still has to go to University. Can their relationship survive the distance on top of everything else between them, or is it time to admit they made a mistake?

  • Amazon Sales Rank: #144232 in eBooks
  • Published on: 2015-09-15
  • Released on: 2015-09-15
  • Format: Kindle eBook

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